Tag: breakthrough

Coli by CRISPR and CRISPRi methods toward the production of a chemical known as 1,4-butanediol, which is utilized in fiber manufacturing. In order to provide 1,4-butanediol, the authors alter the metabolic regulation of the Escherichia coli by CRISPR to induce level mutation in the gltA gene, knockout of the unhappy gene, and knock-in six genes… Read more